Fig. 4. GA modulates instant autophagic flux upon UV-B -irradiation in HDFs. GA pre-treatment to HDFs modulates UV-B induced autophagic flux at initial time points (3 to 6 h post-irradiation of UV-B), (Fig. 4A to 4D). UV-B induced autophagy in response to oxidative stress-mediated manifestation of ER stress is significantly upregulated in GA pre-treated HDFs as is clear from western blotting analysis of P62 (Fig. 4A), Becn1 (Fig. 4B), mTOR (Fig. 4C) & agt7 (Fig. 4D) in 3 h post-irradiation up to 6 h, (**p<0.01 as compared to UV-B only), (*p<0.05 as compared to GA treated). Immunofluorescence of P62 protein in 6 h post-irradiation also suggests modulation of UV-B induced autophagic flux in GA treated HDFs, (Fig. 4E & 4F), (**p<0.01 as compared to both UV-B & GA treated, (*#p<0.001 as compared to BAF A1 treated).